ABSTRACT
To achieve sustainable resources use of Polygonum multiflorum, adventitious roots were efficiently induced and cultured by suspension culture. In order to obtain optimal medium for induction adventitious roots from the young stems of P. multiflorum, MS medium was optimized by supplementing with different concentrations of sucrose and plant growth substances. The optimal medium for suspension culture of adventitious roots was determined by orthogonal design. The adventitious roots with suspension culture were subcultured, and the growth curve was also determined. Furthermore, the effective compound in adventitious roots was detected. The result indicated that the optimal medium for efficient induction of adventitious roots was MS medium containing 4% w/v sucrose, supplemented with 2.0 mg x L(-1) NAA, and 0.2 mg x L(-1) 6-BA. The optimal medium for suspension culture of adventitious roots was MS medium containing 3% sucrose, supplemented with 2.0 mg x L(-1) NAA, and 0.2 mg x L(-1) ABT-7.2,3,5,4'-tetrahydroxyl-diphenyl-ethylene-2-O-beta-D-glucoside was detected in adventitious roots, which was effective compound in medicinal material of P. multiflorum. In conclusion, the experiment achieved efficient induction and suspension culture of adventitious roots of P. multiflorum, and laid a foundation for the research on the sustainable use of traditional Chinese medicine resources.
Subject(s)
Culture Media , Chemistry , Metabolism , Plant Growth Regulators , Metabolism , Plant Roots , Metabolism , Polygonaceae , Metabolism , Sucrose , Metabolism , Tissue Culture Techniques , MethodsABSTRACT
<p><b>OBJECTIVE</b>To establish and optimize the rapid propagation system of Polygonum multiflorum, as well as explore method for induction and identification of autotetraploid.</p><p><b>METHOD</b>Propagation medium was optimized by orthogonal test. The buds were immersed in colchicine solution with different concentrations for different time to select induction conditions for autotetraploid of P. multiflorum.</p><p><b>RESULT</b>The most appropriate propagation medium was MS medium supplemented with 1.0 mg x L(-1) 6-BA, 0.3 mg x L(-1) NAA, and 0.4 mg x L(-1) PP333. That the buds were soaked in 0.2% colchicine solution for 30 h, or soaked in 0.3% colchicine solution for 18 h, was optimal condition to induce autopolyploid of P. multiflorum with induction rate as high as 16.7%.</p><p><b>CONCLUSION</b>Rapid propagation of P. multiflorum could be achieved by tissue culture. Furthermore, colchicine was an effective inducer of polyploidy, and 25 tetraploid lines were obtained through chromosome identification. The experiment laid a foundation for the wild resource conservation, superior varieties breeding of P. multiflorum.</p>
Subject(s)
Chromosomes, Plant , Genetics , Culture Media , Metabolism , Polygonum , Genetics , Metabolism , Tetraploidy , Tissue Culture Techniques , MethodsABSTRACT
<p><b>OBJECTIVE</b>To establish a chromatographic fingerprint of Lonicera japonica and apply it to identify geo-authentic of L. japonica.</p><p><b>METHOD</b>The HPLC was applied in chromatographic separation and data were analysed by "computed aided similarity evaluation" software.</p><p><b>RESULT</b>There exited distinct difference of chemical components group between and non-authentic samples.</p><p><b>CONCLUSION</b>The established HPLC fingerprint can be used for the identification of geo-authentic of L. japonica.</p>